Lykakis J J, Cox F E
Immunology. 1968 Sep;15(3):429-37.
Toads, , were immunized with the ciliate and their sera examined for specific anti- precipitating, immobilizing, agglutinating and lytic antibodies 65–75 days later. Precipitating antibodies were detected in whole antiserum by ring and Ouchterlony tests against soluble antigens of . Immobilizing, agglutinating and lytic antibodies against living ciliates were also detected in whole serum. Antibody activity was localized in the serum fraction corresponding with mammalian γ-globulin after starch block electrophoresis and this was confirmed by immunoelectrophoresis. After fractionation on Sephadex G-200 precipitating antibodies were found to correspond with γG immunoglobulins and non-precipitating antibodies with both γM and γG. These results are comparable with the situation which exists in rabbits immunized against .
用纤毛虫对蟾蜍进行免疫，并在65至75天后检测它们的血清中特异性抗沉淀、抗固定、抗凝集和溶菌抗体。通过针对纤毛虫可溶性抗原的环状试验和双向琼脂扩散试验在全抗血清中检测到沉淀抗体。在全血清中也检测到针对活纤毛虫的固定、凝集和溶菌抗体。淀粉区带电泳后，抗体活性定位于与哺乳动物γ球蛋白相对应的血清组分中，免疫电泳证实了这一点。在Sephadex G - 200上进行分级分离后，发现沉淀抗体与γG免疫球蛋白相对应，非沉淀抗体与γM和γG均有关。这些结果与用纤毛虫免疫的兔子的情况相当。
