Robrish S A, Kemp C W, Bowen W H
Appl Environ Microbiol. 1978 Apr;35(4):743-9. doi: 10.1128/aem.35.4.743-749.1978.
The viable cell mass in plaque samples obtained from monkeys was estimated by determining the concentration of extractable adenosine triphosphate (ATP), and total cell mass was estimated by measuring the protein content. The results were expressed in terms of the specific ATP and protein contents of Streptococcus sanguis. The viable counts estimated by these techniques were comparable to or exceeded viable counts obtained by other investigators using conventional bacteriological methods.
通过测定可提取的三磷酸腺苷(ATP)浓度来估计从猴子身上获取的菌斑样本中的活细胞量,并通过测量蛋白质含量来估计总细胞量。结果以血链球菌的特定ATP和蛋白质含量表示。通过这些技术估计的活菌数与其他研究人员使用传统细菌学方法获得的活菌数相当或超过后者。