Xia Shangming, Gao Xiang, Sun Kangyuan, Liang Fei, Jiang Wenbin, Leng Yanqiu
Weifang Total Pollutant Discharge Control Center, Weifang 261061, China.
Weifang Ecological Environment Monitoring Center, Weifang 261061, China.
Anal Methods. 2026 Mar 2. doi: 10.1039/d6ay00104a.
Zearalenone (ZEA), an environmental mycotoxin produced by fungi, is widely present in grains and grain products. Due to its potent carcinogenicity and toxicity, it poses a serious threat to human and animal health, necessitating the development of simple, sensitive, and reliable ZEA detection methods. This study constructed a novel electrochemiluminescence (ECL) probe based on CuO@Au. This probe synergistically interacts with luminol, utilizing enzyme-catalyzed glucose decomposition to generate HO, which is further catalyzed to hydroxyl radicals (OH˙), thereby significantly amplifying the ECL signal. To enhance catalytic efficiency, a Tb-Cu metal-organic framework (Tb-Cu MOF) with horseradish peroxidase-mimetic activity was integrated onto the sensor interface to accelerate hydrogen peroxide decomposition. This integrated sensing platform achieves highly sensitive detection of ZEA, with a detection limit as low as 0.017 pg mL and a linear range spanning 0.10 pg mL to 100 ng mL. This strategy provides a powerful analytical tool for detecting trace toxins in complex biological matrices, demonstrating broad application prospects in fields such as food safety and environmental analysis.
玉米赤霉烯酮（ZEA）是一种由真菌产生的环境霉菌毒素，广泛存在于谷物及谷物制品中。由于其强大的致癌性和毒性，对人类和动物健康构成严重威胁，因此需要开发简单、灵敏且可靠的ZEA检测方法。本研究构建了一种基于CuO@Au的新型电化学发光（ECL）探针。该探针与鲁米诺协同作用，利用酶催化葡萄糖分解产生H₂O₂，H₂O₂进一步被催化生成羟基自由基（OH˙），从而显著放大ECL信号。为提高催化效率，将具有辣根过氧化物酶模拟活性的Tb-Cu金属有机框架（Tb-Cu MOF）整合到传感器界面以加速过氧化氢分解。这种集成传感平台实现了对ZEA的高灵敏检测——检测限低至0.017 pg/mL，线性范围为0.10 pg/mL至100 ng/mL。该策略为检测复杂生物基质中的痕量毒素提供了强大的分析工具，在食品安全和环境分析等领域展现出广阔的应用前景。
