Baxter J H
J Lipid Res. 1968 Sep;9(5):636-41.
This study was made to determine the extent of absorption of chlorophyll phytol from the intestine of man, and the importance of chlorophyll as a source of the phytanic acid that accumulates in Refsum's disease. Uniformly (14)C-labeled pheophytin a (the Mg-free derivative of chlorophyll a) was fed to normal human subjects and to patients with Refsum's disease. Feces were collected and analyzed. In all subjects, 90-95% of the administered radioactivity was recovered in the feces, still largely in the form of pheophytin a. The phytol radioactivity recovered in the feces averaged about 95% of that in the administered material, which indicates that there had been little absorption of the phytol moiety. Similarly, after 250 g of cooked spinach had been fed to a normal subject, almost the entire phytol content was found in the feces. Less than 5% of the ingested spinach phytol was accounted for in the thoracic duct lymph of another subject. It was concluded that not more than about 5% of the ingested chlorophyll phytol is absorbed by man, whether normal or afflicted with Refsum's disease. On this basis we conclude that the major portion of the phytanic acid that accumulates in Refsum's disease could not be derived from dietary chlorophyll.
本研究旨在确定叶绿素叶绿醇在人体肠道中的吸收程度，以及叶绿素作为累积在雷夫叙姆病中的植烷酸来源的重要性。将均匀标记有（14）C的脱镁叶绿素a（叶绿素a的无镁衍生物）喂给正常人类受试者和雷夫叙姆病患者。收集并分析粪便。在所有受试者中，90 - 95%的给药放射性在粪便中回收，仍主要以脱镁叶绿素a的形式存在。粪便中回收的叶绿醇放射性平均约为给药物质中放射性的95%，这表明叶绿醇部分几乎没有吸收。同样，在将250克煮熟的菠菜喂给一名正常受试者后，几乎所有的叶绿醇都在粪便中被发现。在另一名受试者的胸导管淋巴中，摄入的菠菜叶绿醇不到5%。得出的结论是，无论正常与否，人体摄入的叶绿素叶绿醇被吸收的比例不超过约5%。在此基础上，我们得出结论，雷夫叙姆病中累积的植烷酸的主要部分不可能来自膳食叶绿素。
