Baxter J H, Steinberg D
J Lipid Res. 1967 Nov;8(6):615-20.
The fate of ingested chlorophyll-particularly of the phytol portion of the molecule-was studied. Uniformly (14)C-labeled pheophytin a (the Mg-free derivative of chlorophyll a) was prepared from an extract of tobacco leaves grown in (14)CO(2), and was administered by stomach tube to rats in which the thoracic duct had been cannulated. Only about 2% of the administered radioactivity was absorbed in 24 hr, largely into the thoracic duct lymph. Moreover, only a fraction of this lymph radioactivity was derived from phytol (i.e., was found in phytol, phytenic acid, or phytanic acid). The results indicated that not more than 1-2% of chlorophyll phytol is available for absorption by the rat. Similarly, after the administration of whole spinach or spinach extract (not labeled) to rats, only about 1% of the total phytol content was absorbed into the intestinal lymph. Nearly all of the administered phytol was found in the feces and the contents of the colon, and was still largely in the form of pheophytin. The study also indicated that little of the nonphytol portion of the chlorophyll molecule is absorbed.
研究了摄入的叶绿素的命运，特别是分子中叶绿醇部分的命运。用在(^{14}CO_2)中生长的烟草叶提取物制备了均匀标记有(^{14}C)的脱镁叶绿素a（叶绿素a的无镁衍生物），并通过胃管给胸导管已插管的大鼠给药。在24小时内，仅约2%的给药放射性被吸收，大部分进入胸导管淋巴。此外，该淋巴放射性中只有一小部分来自叶绿醇（即在叶绿醇、植烷酸或植酸中发现）。结果表明，大鼠可吸收的叶绿素叶绿醇不超过1 - 2%。同样，给大鼠喂食全菠菜或菠菜提取物（未标记）后，肠道淋巴中仅吸收了约1%的总叶绿醇含量。几乎所有给药的叶绿醇都存在于粪便和结肠内容物中，并且大部分仍以脱镁叶绿素的形式存在。该研究还表明，叶绿素分子的非叶绿醇部分很少被吸收。
