Scaff W L, Dyer D L, Mori K
J Bacteriol. 1969 Apr;98(1):246-8. doi: 10.1128/jb.98.1.246-248.1969.
The europium chelate of 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione (thenoyl-trifluoroacetone; TTA) is firmly bound to microorganisms. It fluoresces brightly at 613 nm with activation at 340 nm. Cells may be stained with 10(-3)m chelate in 50% ethyl alcohol, followed by washing with 50% ethyl alcohol. Equal or better stains are produced with 10(-3)m aqueous europium salt, water wash, and 10(-2)m aqueous TTA. A noncomplexing buffer should be used to maintain the pH at 6.5 to 6.8.
4,4,4-三氟-1-(2-噻吩基)-1,3-丁二酮（噻吩甲酰三氟丙酮；TTA）的铕螯合物与微生物紧密结合。它在340nm激发下于613nm处发出明亮荧光。细胞可用50%乙醇中的10⁻³m螯合物染色，随后用50%乙醇洗涤。用10⁻³m铕盐水溶液、水洗以及10⁻²m TTA水溶液可产生同等或更好的染色效果。应使用非络合缓冲液将pH维持在6.5至6.8。
