Tanaka Y
J Cell Biol. 1969 May;41(2):424-30. doi: 10.1083/jcb.41.2.424.
With pinacyanol as the supravital stain, a preferential effect on mitochondria of KB cells was achieved by the irradiation with the ruby laser beam. The observation confirmed the results of other workers using janus green B in the same experimental system. The preferential effect on mitochondria was noted in the area extending 8-10 micro beyond the nonpreferential damage of 4-5 micro in diameter. The opaque material associated with mitochondria possibly represented coagulated protein. The effect involved cristae mitochondriales without severe disarrangement of their structure. The opaque material could be interpreted as the result of direct interaction between mitochondria and the laser beam, even though the mitochondria were noted outside of the previously estimated focal spot size of about 3 micro Within the thickness of 2-4 micro of monolayered cells, larger areas of damage can be accounted for by divergence of the beam which is focused by a microscope objective of very short focal length. A threshold of biologic effectiveness is probably also involved.
以藻青素作为超活染色剂，用红宝石激光束照射可对KB细胞的线粒体产生优先效应。该观察结果证实了其他研究人员在同一实验系统中使用健那绿B所得到的结果。在直径4 - 5微米的非优先损伤范围之外8 - 10微米的区域内，观察到了对线粒体的优先效应。与线粒体相关的不透明物质可能代表凝固的蛋白质。这种效应涉及线粒体嵴，但其结构没有严重紊乱。即使在线粒体位于先前估计的约3微米焦斑尺寸之外时也观察到了这种情况，该不透明物质可被解释为线粒体与激光束直接相互作用的结果。在单层细胞2 - 4微米的厚度范围内，更大面积的损伤可能是由通过极短焦距显微镜物镜聚焦的光束发散所致。可能还涉及生物效应的阈值。
