Sequential immunofluorescent staining: a simple and useful technique.

Different antigens in tissue sections can be revealed by sequential application of corresponding fluorescein-labelled antisera. This is achieved by destroying the fluorochrome with the aid of ultraviolet light. The usefulness of the method is illustrated by analyses of sections from lymphoid tissue of mice immunized with ferritin. Anti-ferritin producing cells are identified as to their immunoglobulin class by sequential incubation with labelled specific antisera directed against mouse IgA, IgM, IgG and IgG.