De Siqueira A F, Ribeiro R D, Ferriolli Filho F
Rev Bras Pesqui Med Biol. 1978 May;11(1):35-7.
From rats infected with the Y strain of T. cruzi trypomastigotes were recovered in successive days to be used in agglutination tests (AT) and in direct immunofluorescence tests (DIT). Titres obtained in AT reached 1/640 with trypomastigotes in the fourth and fifth days, decreased to 1/160 in the sixth day and were negative in the seventh day; in the tenth day the titers were again up to 1/40 and reached 1/320 in the eleventh day. The DIT were negative with trypomastigotes in the fourth and fifth days, a few were positive in the sixth day and numerous were positive in the seventh day; again a few were positive in the tenth day and all were negative in the eleventh day. These results seems to indicate that the trypomastigotes are blocked by circulating antibodies when they are not agglutinated by immune sera in vitro.
从感染克氏锥虫Y株的大鼠体内，在连续数天回收锥鞭毛体，用于凝集试验（AT）和直接免疫荧光试验（DIT）。在AT中，用第4天和第5天的锥鞭毛体获得的滴度达到1/640，在第6天降至1/160，在第7天为阴性；在第10天滴度再次高达1/40，并在第11天达到1/320。DIT在第4天和第5天用锥鞭毛体检测为阴性，在第6天少数为阳性，在第7天多数为阳性；在第10天再次少数为阳性，在第11天全部为阴性。这些结果似乎表明，当锥鞭毛体在体外不被免疫血清凝集时，它们会被循环抗体阻断。
