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混合培养细胞致病性：一种检测小鼠白血病病毒在组织培养中生长情况的新试验。

Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture.

作者信息

Klement V, Rowe W P, Hartley J W, Pugh W E

出版信息

Proc Natl Acad Sci U S A. 1969 Jul;63(3):753-8. doi: 10.1073/pnas.63.3.753.

DOI:10.1073/pnas.63.3.753
PMID:4186808
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC223516/
Abstract

Tissue culture cells of a Rous virus-induced rat tumor undergo syncytium formation when placed in contact with mouse embryo cell cultures infected with murine leukemia viruses. This phenomenon can be used as a cytopathic end point for isolating and titrating these viruses in tissue culture. The principle should be applicable to detection of leukemia viruses of other species.

摘要

劳斯病毒诱发的大鼠肿瘤的组织培养细胞，当与感染鼠白血病病毒的小鼠胚胎细胞培养物接触时，会发生合胞体形成。这种现象可作为在组织培养中分离和滴定这些病毒的细胞病变终点。该原理应适用于检测其他物种的白血病病毒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/223516/484356968882/pnas00109-0186-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/223516/f2d0b675bcd5/pnas00109-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/223516/99275f2aabea/pnas00109-0185-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/223516/cdd6955d7cb2/pnas00109-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/223516/484356968882/pnas00109-0186-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/223516/f2d0b675bcd5/pnas00109-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/223516/99275f2aabea/pnas00109-0185-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/223516/cdd6955d7cb2/pnas00109-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/223516/484356968882/pnas00109-0186-b.jpg

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本文引用的文献

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Demonstration of the absence of infectious Rous virus in rat tumour XC, whose structurally intact cells produce Rous sarcoma when transferred to chicks.在大鼠肿瘤XC中未检测到传染性劳氏病毒，该肿瘤结构完整的细胞转移至雏鸡时会产生劳氏肉瘤。
Folia Biol (Praha). 1963 Apr;9:77-81.
2
Presence of chicken tumour virus in the sarcoma of the adult rat inoculated after birth with Rous sarcoma tissue.出生后接种劳斯肉瘤组织的成年大鼠肉瘤中鸡肿瘤病毒的存在情况。
Nature. 1960 Jun 18;186:980-1. doi: 10.1038/186980b0.
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Biological and serological properties of viral particles from a nonproducer rat neoplasm induced by murine sarcoma virus (Moloney).
Viruses. 2018 Apr 18;10(4):203. doi: 10.3390/v10040203.
4
Gamma-interferon-inducible, lysosome/endosome-localized thiolreductase, GILT, has anti-retroviral activity and its expression is counteracted by HIV-1.γ干扰素诱导的、定位于溶酶体/内体的硫醇还原酶GILT具有抗逆转录病毒活性，其表达受到HIV-1的抑制。
Oncotarget. 2016 Nov 1;7(44):71255-71273. doi: 10.18632/oncotarget.12104.
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In vivo imaging of virological synapses.活细胞内病毒突触的成像。
Nat Commun. 2012;3:1320. doi: 10.1038/ncomms2338.
6
Retroviruses can establish filopodial bridges for efficient cell-to-cell transmission.逆转录病毒可以建立丝状伪足桥以实现高效的细胞间传播。
Nat Cell Biol. 2007 Mar;9(3):310-5. doi: 10.1038/ncb1544. Epub 2007 Feb 11.
7
Kinetic analyses of the surface-transmembrane disulfide bond isomerization-controlled fusion activation pathway in Moloney murine leukemia virus.莫洛尼鼠白血病病毒中表面跨膜二硫键异构化控制的融合激活途径的动力学分析。
J Virol. 2005 Nov;79(22):13856-64. doi: 10.1128/JVI.79.22.13856-13864.2005.
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Actin- and myosin-driven movement of viruses along filopodia precedes their entry into cells.病毒在丝状伪足上由肌动蛋白和肌球蛋白驱动的移动先于其进入细胞。
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