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Acta Vet Scand. 1969;10(2):118-26. doi: 10.1186/BF03548283.
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[A study on serum protein fraction by cellulose acetate membrane electrophoresis in childhood].[儿童期醋酸纤维素薄膜电泳血清蛋白组分的研究]
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本文引用的文献

1
A PREPARATIVE METHOD FOR THE SEPARATION OF 7S GAMMA GLOBULIN FROM HUMAN SERUM.一种从人血清中分离7Sγ球蛋白的制备方法。
Arch Biochem Biophys. 1964 Dec;108:514-22. doi: 10.1016/0003-9861(64)90436-9.
2
Chemical and electrophoretic studies of the serums of cattle infected naturally with Asia-1 type foot-and-mouth disease virus.对自然感染亚洲1型口蹄疫病毒的牛血清进行的化学和电泳研究。
Am J Vet Res. 1961 Mar;22:217-23.
3
The analysis of serum proteins of domestic animals by filter-paper electrophoresis; a review.
Cornell Vet. 1959 Jan;49(1):82-96.
4
A cellulose acetate supporting medium for zone electrophoresis.一种用于区带电泳的醋酸纤维素支持介质。
Clin Chim Acta. 1957 Aug;2(4):297-303. doi: 10.1016/0009-8981(57)90005-0.
5
Buffer composition in paper electrophoresis; considerations on its influence, with special reference to the interaction between small ions and proteins.纸电泳中的缓冲液组成；关于其影响的考量，特别提及小离子与蛋白质之间的相互作用
Clin Chem. 1956 Apr;2(2):99-111.
6
Highly purified antithrombin 3 with heparin cofactor activity prepared by disc electrophoresis.通过圆盘电泳制备的具有肝素辅因子活性的高度纯化抗凝血酶3。
Scand J Clin Lab Invest. 1968;21(1):89-91. doi: 10.3109/00365516809076981.
7
The electrophoretic pattern of serum proteins in normal animals.正常动物血清蛋白的电泳图谱。
Res Vet Sci. 1967 Apr;8(2):137-42.

健康动物牛血清蛋白的醋酸纤维素薄膜电泳研究。

Studies on electrophoresis on cellulose acetate membrane of bovine serum proteins in healthy animals.

作者信息

Ek N

出版信息

Acta Vet Scand. 1969;10(2):118-26. doi: 10.1186/BF03548283.

DOI:10.1186/BF03548283
PMID:4187674
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8741107/
Abstract

A method for rapid electrophoresis of serum proteins on a cellulose acetate membrane is described. In order to experimentally identify the various larger globulin fractions in normal bovine serum, pure bovine y-globulin and a bovine attraction (Antitrombin III) were used. These fractions were electrophoretically compared with bovine serum and the various fractions on the diagram determined. A diluted veronal buffer with added Ga-acetate gave considerably better separation than the reported standard buffer. In the last part of the experiment normal percentage values for the composition of the protein fractions in bovine serum were calculated by the method described. The material consisted of 50 cows of the N.R.F. breed. The values are compared with corresponding figures reported by other authors, and the results are discussed.

摘要

本文描述了一种在醋酸纤维素膜上快速电泳血清蛋白的方法。为了通过实验鉴定正常牛血清中各种较大的球蛋白组分，使用了纯牛γ-球蛋白和一种牛抗凝血酶（抗凝血酶III）。将这些组分与牛血清进行电泳比较，并确定图上的各种组分。添加了醋酸镓的稀释巴比妥缓冲液比报道的标准缓冲液分离效果要好得多。在实验的最后部分，采用所述方法计算了牛血清中蛋白质组分组成的正常百分比值。材料由50头N.R.F.品种的奶牛组成。将这些值与其他作者报道的相应数据进行比较，并对结果进行了讨论。