哺乳大鼠肠道中蔗糖酶和乳糖酶的合成对底物给药的反应。
Sucrase and lactase synthesis in suckling rat intestine in response to substrate administration.
作者信息
Raul F, Simon P M, Kedinger M, Grenier J F, Haffen K
出版信息
Biol Neonate. 1978;33(1-2):100-5. doi: 10.1159/000241057.
The intestinal brush border disaccharidases separated by gel electrophoresis were studied after oral administration of a high sucrose or lactose diet to 11-day-old suckling rats during 3 days. Some modifications of the brush border protein and eyzyme patterns could be attributed to the effect of the basic diet: increase of glucoamylase, appearance of a weak sucrase activity and of a second molecular form of maltase. However, the specific action of a given disaccharide on the synthesis of the corresponding hydrolytic enzyme could be clearly demonstrated. Indeed, the electrophoretic pattern after sucrose or lactose feeding showed a marked increase of the protein bands corresponding to sucrase-isomaltase or lactase activities.
对11日龄的乳鼠连续3天经口给予高蔗糖或乳糖饮食后，研究了通过凝胶电泳分离的肠刷状缘双糖酶。刷状缘蛋白和酶模式的一些改变可归因于基础饮食的影响：葡糖淀粉酶增加、出现弱蔗糖酶活性以及麦芽糖酶的第二种分子形式。然而，可以清楚地证明特定双糖对相应水解酶合成的具体作用。实际上，喂食蔗糖或乳糖后的电泳图谱显示，对应于蔗糖酶-异麦芽糖酶或乳糖酶活性的蛋白条带显著增加。
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