Goda T, Bustamante S
J Pediatr Gastroenterol Nutr. 1985 Dec;4(6):998-1008. doi: 10.1097/00005176-198512000-00024.
To characterize the mechanisms leading to dietary evoked increases of lactase and sucrase activities by carbohydrates, we performed a quantitative comparison of the effects of lactose and sucrose on the corresponding disaccharidases in the jejunum of 2-month-old rats. For 7-10 days the rats were fed a low-starch (5 cal%), high-fat (73 cal%) diet, and for various periods of time (3-72 h) were given an isocaloric sucrose or lactose (20, 40, or 70 cal%) diet. Lactase and sucrase activities in jejunal homogenates were significantly increased within 3 h after the initial feeding of the sucrose (40 cal%) diet. After 3 h of feeding the sucrose diet, sucrase activity gradually increased and reached its maximum at 24 h, whereas lactase activity did not exhibit further change. Increased intake of sucrose led to an increase of lactase and sucrase activity. Within a range of doses of digestible amounts of lactose, the effect of diet containing lactose on these disaccharidase activities was similar to the effect of the diet containing sucrose. This similarity suggests the important role of the common constituent sugar, i.e., glucose. Further, analyses of response to these disaccharides along the villus-crypt axis revealed that the increase of lactase activity occurs at a more apical and broader locus of cohort of epithelial cells along the height of the villus than that of sucrase, suggesting that different mechanisms are involved in dietary regulation of lactase and sucrase.
为了阐明碳水化合物引起饮食诱发乳糖酶和蔗糖酶活性增加的机制，我们对乳糖和蔗糖对2月龄大鼠空肠中相应二糖酶的影响进行了定量比较。大鼠食用低淀粉（5千卡%）、高脂肪（73千卡%）饮食7 - 10天，然后在不同时间段（3 - 72小时）给予等热量的蔗糖或乳糖（20、40或70千卡%）饮食。在开始喂食蔗糖（40千卡%）饮食后3小时内，空肠匀浆中的乳糖酶和蔗糖酶活性显著增加。喂食蔗糖饮食3小时后，蔗糖酶活性逐渐增加，并在24小时达到最大值，而乳糖酶活性没有进一步变化。蔗糖摄入量增加导致乳糖酶和蔗糖酶活性增加。在可消化量的乳糖剂量范围内，含乳糖饮食对这些二糖酶活性的影响与含蔗糖饮食的影响相似。这种相似性表明共同成分糖即葡萄糖的重要作用。此外，沿着绒毛 - 隐窝轴对这些二糖反应的分析表明，乳糖酶活性的增加发生在沿绒毛高度上皮细胞群体更顶端且更广泛的位置，比蔗糖酶的位置更高，这表明乳糖酶和蔗糖酶的饮食调节涉及不同机制。
