Mitruka B M, Jonas A M
Appl Microbiol. 1969 Dec;18(6):1072-6. doi: 10.1128/am.18.6.1072-1076.1969.
Rats inoculated with Streptococcus faecalis developed endocarditis and demonstrated a 6- to 30-fold increase in aldolase, isocitric dehydrogenase, phosphohexose isomerase, and lactic dehydrogenase. The animals infected with Bacillus subtilis did not develop overt disease nor significant increases in enzyme activities, but viable organisms were recovered at 2 weeks. Rats inoculated with mixed culture of these organisms showed a 2- to 10-fold increase of enzyme activities without evidence of pathological anatomic changes. Both organisms were recovered at necropsy. The total protein and glycoproteins followed the patterns of enzyme activities. There were major changes in alpha(1), alpha(2), and beta globulins and glycoglobuulins at the early stages of infection. The protein-bound hexose changes coincided with the severity of S. faecalis infection, but were at normal levels after 72 hr of infection of B. subtilis and S. faecalis mixed infections. The results indicate that B. subtilis infection modified the pathogenicity of S. faecalis and by an unknown mechanism affected protein and glycoprotein production in serum of experimental rats.
接种粪肠球菌的大鼠发生了心内膜炎，其醛缩酶、异柠檬酸脱氢酶、磷酸己糖异构酶和乳酸脱氢酶增加了6至30倍。感染枯草芽孢杆菌的动物未出现明显疾病，酶活性也未显著增加，但在2周时可分离出活的微生物。接种这两种微生物混合培养物的大鼠酶活性增加了2至10倍，且无病理解剖学变化的证据。尸检时两种微生物均可被分离出来。总蛋白和糖蛋白呈现出与酶活性相同的模式。在感染早期，α(1)、α(2)和β球蛋白以及糖球蛋白有主要变化。蛋白结合己糖的变化与粪肠球菌感染的严重程度一致，但在枯草芽孢杆菌和粪肠球菌混合感染72小时后处于正常水平。结果表明，枯草芽孢杆菌感染改变了粪肠球菌的致病性，并通过未知机制影响了实验大鼠血清中蛋白质和糖蛋白的产生。
