巨大芽孢杆菌多分散闭环脱氧核糖核酸分子的特性分析
Characterization of the polydisperse closed circular deoxyribonucleic acid molecules of Bacillus megaterium.
作者信息
Henneberry R C, Carlton B C
出版信息
J Bacteriol. 1973 May;114(2):625-31. doi: 10.1128/jb.114.2.625-631.1973.
Abstract
The polydisperse circular deoxyribonucleic acid (DNA) molecules which comprise up to 30% of the total extractable DNA of Bacillus megaterium strain 216 have been purified and partially characterized. Banding in cesium chlorideethidium bromide by "gradient relaxation" in a fixed-angle rotor provided good resolution of circular and chromosomal DNAs for preparative separations. Renaturation studies on purified circular DNA failed to reveal a rapidly renaturing fraction, and DNA-DNA hybridization studies indicated that the majority of the chromosomal nucleotide sequences are represented in the heterogeneous-size population of circular molecules. It is concluded that the circular DNA of B. megaterium does not represent typical bacterial plasmid DNA. The possibility that the circular DNA molecules are the result of the expression of a defective bacteriophage is discussed.
摘要
巨大芽孢杆菌216株中占总可提取DNA达30%的多分散环状脱氧核糖核酸（DNA）分子已被纯化并进行了部分特性鉴定。在固定角度转子中通过“梯度弛豫”在氯化铯-溴化乙锭中进行条带分离，为制备性分离提供了环状和染色体DNA的良好分辨率。对纯化的环状DNA进行复性研究未能揭示快速复性部分，DNA-DNA杂交研究表明，大多数染色体核苷酸序列存在于环状分子的异质大小群体中。得出结论，巨大芽孢杆菌的环状DNA不代表典型的细菌质粒DNA。讨论了环状DNA分子是缺陷噬菌体表达结果的可能性。
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