Lindberg M, Novick R P
J Bacteriol. 1973 Jul;115(1):139-45. doi: 10.1128/jb.115.1.139-145.1973.
Transformation of Staphylococcus aureus cells with circular duplex deoxyribonucleic acid prepared from plasmid-carrying strains by alkali denaturation and selective renaturation or by dye-buoyant density centrifugation is reported. In all of the transformants tested, the transformed markers became established as autonomous plasmids that were biologically and physically indistinguishable from those carried by the donor strains. Transformation with bulk deoxyribonucleic acid from a strain carrying the penicillinase plasmid, PI(258), gave rise to transformants in which the erythromycin locus, the only plasmid marker transformed, was shown to be integrated into the host chromosome.
据报道，通过碱变性和选择性复性或通过溴化乙锭-密度梯度离心法，用从携带质粒的菌株制备的环状双链脱氧核糖核酸对金黄色葡萄球菌细胞进行转化。在所有测试的转化体中，转化的标记物成为自主质粒，在生物学和物理性质上与供体菌株携带的质粒无法区分。用携带青霉素酶质粒PI(258)的菌株的大量脱氧核糖核酸进行转化，产生了转化体，其中唯一被转化的质粒标记物红霉素位点被证明整合到宿主染色体中。
