利用脐血清免疫吸附剂对人抗IgM血清进行免疫特异性纯化。
Immunospecific purification of human anti-IgM serum utilizing cord serum immunoadsorbent.
作者信息
Shinkov D, Eskenazy M
出版信息
Immunology. 1973 Apr;24(4):679-82.
Abstract
An immunoadsorbent was prepared from cord serum by crosslinking with glutaraldehyde. Rabbit anti-IgM serum raised against the euglobulin fraction of Waldenström macroglobulinaemia serum was purified to a considerable degree from antibodies to extraneous proteins following immunoadsorption. The adsorbed serum contained predominantly antibodies of IgM specificity.
摘要
通过与戊二醛交联，从脐带血清制备了一种免疫吸附剂。用瓦尔登斯特伦巨球蛋白血症血清的优球蛋白部分免疫家兔产生的抗IgM血清，经免疫吸附后，从针对外来蛋白质的抗体中得到了相当程度的纯化。吸附后的血清主要含有IgM特异性抗体。
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本文引用的文献
1
CHARACTERIZATION OF A HUMAN MACROGLOBULIN. I. THE MOLECULAR WEIGHT OF ITS SUBUNIT.一种人巨球蛋白的特性。I. 其亚基的分子量
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Ultrastructure of papain and pepsin digestion fragments of human IgM globulins.人免疫球蛋白M木瓜蛋白酶和胃蛋白酶消化片段的超微结构
J Exp Med. 1969 Oct 1;130(4):691-705. doi: 10.1084/jem.130.4.691.
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