Burgess A W, Momany F A, Scheraga H A
Proc Natl Acad Sci U S A. 1973 May;70(5):1456-60. doi: 10.1073/pnas.70.5.1456.
Conformational energy calculations on thyrotropin releasing factor and on several of its analogues indicate that the central histidyl residue of the native molecule is in an extended conformation. Some derivatives (with a reduced biological activity) have an altered conformation. Since some substitutions leave the conformation unchanged but alter the biological activity, these substitutions must involve the sites responsible for binding of the hormone to its receptor.
对促甲状腺激素释放因子及其几种类似物的构象能量计算表明，天然分子的中心组氨酸残基处于伸展构象。一些衍生物（生物活性降低）具有改变的构象。由于一些取代使构象保持不变但改变了生物活性，这些取代必定涉及负责激素与其受体结合的位点。
