细胞黏菌盘基网柄菌细胞外糖苷酶的纯化及性质
The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum.
作者信息
Every D, Ashworth J M
出版信息
Biochem J. 1973 May;133(1):37-47. doi: 10.1042/bj1330037.
Abstract
The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous preparations whereas the alpha-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.
摘要
本文描述了从盘基网柄菌Ax-2株变形体的用过的生长培养基中纯化β-N-乙酰己糖胺酶、α-葡萄糖苷酶、α-甘露糖苷酶和β-葡萄糖苷酶的方法。β-N-乙酰己糖胺酶和α-葡萄糖苷酶以高产量获得且为均一制剂，而α-甘露糖苷酶制剂由两种电泳性质不同的同工酶组成。文中还描述了这些酶的物理、化学和动力学性质。
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HISTOCHEMICAL DEMONSTRATION OF N-ACETYL-BETA-GLUCOSAMINIDASE EMPLOYING NAPHTHOL AS-BI N-ACETYL-BETA -GLUCOSAMINIDE AS SUBSTRATE.以萘酚AS - BI N - 乙酰 - β - 氨基葡萄糖苷为底物的N - 乙酰 - β - 氨基葡萄糖苷酶的组织化学显示
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PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.大肠杆菌K12 β-半乳糖苷酶的纯化、组成及分子量
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