黄绒泡黏菌二倍体细胞和单倍体细胞中的肌动蛋白——与骨骼肌肌动蛋白的比较。

Actin from diploid and haploid cells of the myxomycete Physarum flavicomum--comparison with skeletal muscle actin.

作者信息

Henney H R, Hubbell C

出版信息

Microbios. 1978;23(91):25-34.

PMID:42005

Abstract

Glycerinated diploid microplasmodia of Physarum flavicomum exhibited ATP-dependent contractility typical of actomyosin controlled systems. Actin was isolated from microplasmodia, haploid amoebae-swarm cells, and rabbit skeletal muscle, by classical methods as well as ATP-DEAE cellulose chromatography, and isoelectric focusing. Actins from all three sources had isoelectric points in the pH 6.5-7.0 range, and behaved similarly during the various isolation procedures. SDS-gel electrophoresis revealed that the actin from microplasmodia and muscle had a molecule weight of about 46,000 daltons while the amoebae-swarm cells possessed proteins of molecular weights of about 46,000 and 51,000 daltons.

摘要

淡黄绒泡菌的甘油化二倍体微原质团表现出典型的由肌动球蛋白控制系统控制的ATP依赖性收缩性。通过经典方法以及ATP-DEAE纤维素色谱法和等电聚焦，从微原质团、单倍体变形虫-游动细胞和兔骨骼肌中分离出肌动蛋白。来自所有这三种来源的肌动蛋白的等电点在pH 6.5-7.0范围内，并且在各种分离过程中表现相似。SDS-凝胶电泳显示，来自微原质团和肌肉的肌动蛋白的分子量约为46,000道尔顿，而变形虫-游动细胞拥有分子量约为46,000和51,000道尔顿的蛋白质。