Margulis B A, Podgornaia O I, Galaktionov K I
Biofizika. 1982 Jan-Feb;27(1):87-90.
A method combining advantages of both, charge electrophoresis and isoelectric focusing (IEF) was developed for analysing actins of different tissues. This method was used in investigations of actins from invertebrate muscle sources. Each type of actin has one isoform, pI of which is close to that of the smooth muscle beta-isoactin. Actins from different tissues of the mollusc Spisula s. were identical according to the IEF data.
为分析不同组织的肌动蛋白,开发了一种结合电荷电泳和等电聚焦(IEF)两者优点的方法。该方法用于研究无脊椎动物肌肉来源的肌动蛋白。每种类型的肌动蛋白都有一种同工型,其等电点与平滑肌β-同工肌动蛋白的等电点相近。根据IEF数据,软体动物厚壳贻贝不同组织的肌动蛋白是相同的。