Amprino R
Anat Embryol (Berl). 1979 Jan 30;155(2):135-48. doi: 10.1007/BF00305747.
The right limb buds of chick embryos (H.-H.stages 22 to 26) were transversely divided at approximately the presumptive zeugopod mid-length, and (1) the distal parts exchanged between wing and hind-limb bud in apdv-reversed orientation (operation A), or (2) the severed distal part of the leg bud was reoriented in situ (operation B). Both the proximal and distal segments of the zeugopodal bones developing from the recombined portions of presumptive heterologous territories showed characteristic differences of size and shape in comparison to the corresponding parts of the skeletal pieces of the zeugopod of the intact, control limbs. These developmental changes are interpreted as being the consequence of reciprocal influences exerted by the adjacent parts of mesenchymal territories (or blastemata) that, under normal developmental conditions, are destined to give rise to different bones. Such influences may enhance or depress the growth potential of the skeletogenous populations affected, and, within each cell population, modify the geometry of the growth processes by which the various parts of each skeletal piece attain their typical shape.
将鸡胚（哈勃德-汉密尔顿第22至26期）的右肢芽在大约假定的zeugopod中段长度处横向分割，并且（1）将翅膀和后肢芽之间的远端部分以apdv反转的方向进行交换（操作A），或者（2）将切断的腿芽远端部分在原位重新定向（操作B）。与完整对照肢体zeugopod骨骼部分的相应部位相比，从假定的异源区域的重组部分发育而来的zeugopodal骨骼的近端和远端节段在大小和形状上显示出特征性差异。这些发育变化被解释为间充质区域（或胚基）相邻部分相互影响的结果，在正常发育条件下，这些区域注定会产生不同的骨骼。这种影响可能会增强或抑制受影响的骨骼生成群体的生长潜力，并且在每个细胞群体内，改变每个骨骼部分的各个部分获得其典型形状的生长过程的几何形状。
