Amprino R
Bull Assoc Anat (Nancy). 1979 Jun;63(181):161-5.
By means of various kinds of recombination of the two halves of the chick limb buds transected at the presumptive zeugopod mid-length a continuity was established between the opposite portions of the mesenchymal precursors of heterologous shaft bones of the zeugopod. In later development, both proximal and distal components of the recombined skeletal pieces consistently showed variously marked differences in size and shape in comparison with the corresponding parts of the control bones. Reciprocally, from the opposite portions of the presumptive territory of each zeugopodal bone separated the one from the other so that they could not contact and fuse, hypoplasic pieces developed which failed to attain the size and shape typical of the control segments. Those observations seem to show that morphogenetic interactions reciprocally exerted between the various parts of the presumptive territory of each shaft bone are mandatory to ensure the normal development of these skeletal pieces.
通过将鸡胚肢芽在假定的zeugopod中长度处横切后的两半进行各种重组，在zeugopod异源骨干间充质前体的相对部分之间建立了连续性。在后期发育中，重组骨骼片段的近端和远端成分与对照骨骼的相应部分相比，在大小和形状上始终呈现出各种明显的差异。相反，从每个zeugopodal骨假定区域的相对部分彼此分离，使得它们不能接触和融合，发育出的发育不全的片段未能达到对照节段典型的大小和形状。这些观察结果似乎表明，每个骨干假定区域的各个部分之间相互施加的形态发生相互作用对于确保这些骨骼片段的正常发育是必不可少的。
