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[钝顶螺旋藻生物质中总蛋白分离方法的比较评估]

[Comparative evaluation of methods for isolating total protein from the biomass of Spirulina platensis].

作者信息

Al'bitskaia O N, Zaĭtseva G N, Rogozhin S V, Pakhomova M V, Oshanina N P

出版信息

Prikl Biokhim Mikrobiol. 1979 Jul-Aug;15(4):612-7.

PMID:42047
Abstract

The methods of extracting total protein from the biomass of Spirulina platensis are discussed. The results of studying different procedures of cell wall disruption, soluble protein extraction and precipitation are presented. The best results can be obtained using mechanical disintegration of Spirulina cells for 20 min (at a temperature not higher than 25 degrees C), soluble protein extraction with 0.4% NaOH, and its subsequent precipitation at the isoelectric point with 5% HCl.

摘要

讨论了从钝顶螺旋藻生物质中提取总蛋白的方法。展示了研究细胞壁破碎、可溶性蛋白提取和沉淀的不同程序的结果。通过对钝顶螺旋藻细胞进行20分钟的机械破碎(温度不高于25摄氏度)、用0.4%氢氧化钠提取可溶性蛋白并随后在等电点用5%盐酸沉淀,可获得最佳结果。

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