使用析因设计优化钝顶螺旋藻中藻蓝蛋白的提取
Optimization of phycocyanin extraction from Spirulina platensis using factorial design.
作者信息
Silveira S T, Burkert J F M, Costa J A V, Burkert C A V, Kalil S J
机构信息
Department of Chemistry, Fundação Universidade Federal do Rio Grande, P.O. Box 474, Rio Grande-RS 96201-900, Brazil.
出版信息
Bioresour Technol. 2007 May;98(8):1629-34. doi: 10.1016/j.biortech.2006.05.050. Epub 2006 Sep 8.
Phycocyanin extraction from cyanobacteria Spirulina platensis was optimized using factorial design and response surface techniques. The effects of temperature and biomass-solvent ratio on phycocyanin concentration and extract purity were evaluated to determine the optimum conditions for phycocyanin extraction. The optimum conditions for the extraction of phycocyanin from S. platensis were the highest biomass-solvent ratio, 0.08 gmL(-1), and 25 degrees C. Under these conditions it's possible to obtain an extract of phycocyanin with a concentration of 3.68 mgmL(-1) and purity ratio (A(615)/A(280)) of 0.46.
采用析因设计和响应面技术优化了从钝顶螺旋藻中提取藻蓝蛋白的工艺。评估了温度和生物质与溶剂比例对藻蓝蛋白浓度和提取物纯度的影响，以确定藻蓝蛋白提取的最佳条件。从钝顶螺旋藻中提取藻蓝蛋白的最佳条件是最高的生物质与溶剂比例（0.08 gmL⁻¹）和25℃。在这些条件下，可以获得浓度为3.68 mgmL⁻¹、纯度比（A₆₁₅/A₂₈₀）为0.46的藻蓝蛋白提取物。
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