Mitchell W D, Webb S F, Padmore G R
Ann Clin Biochem. 1979 Jan;16(1):47-50. doi: 10.1177/000456327901600109.
A rapid and simple extraction procedure followed by gas chromatography using a nitrogen detector is described for the analysis of amitriptyline, nortriptyline, imipramine, and desipramine in 100 microliter plasma. No derivitisation of the drugs is required. Recoveries ranged from 93.7 to 104.6%. Within-batch precision and day-to-day variation showed coefficients of variation of less than 10% with the exception of desipramine, for which the day-to-day coefficient of variation was 15.2%. The method was developed to measure plasma concentrations in patients who had taken non-fatal and fatal overdoses of the drugs.
本文描述了一种快速简便的提取方法，随后采用带氮检测器的气相色谱法，用于分析100微升血浆中的阿米替林、去甲替林、丙咪嗪和地昔帕明。无需对药物进行衍生化处理。回收率在93.7%至104.6%之间。批内精密度和日间差异显示，除地昔帕明外，变异系数均小于10%，地昔帕明的日间变异系数为15.2%。该方法用于测量服用了非致命和致命过量这些药物的患者的血浆浓度。
