Caillé J P
Can J Physiol Pharmacol. 1975 Dec;53(6):1178-85. doi: 10.1139/y75-163.
Myoplasmic impedance was measured on a barnacle (Balanus nubilus) single muscle fiber that was placed in a cylindrical cavity to limit the volume and prevent the hydration of the myoplasm. At both ends of the cavity, the myoplasm was in direct contact with an electrolyte solution. When equilibrium with the external medium was reached, the myoplasmic impedance was measured at 10 degrees C with an impedance bridge at 1000 Hz. The results indicated that the myoplasmic impedance of the muscle fiber is mainly resistive. Treating the myoplasm as a suspension of small conductive particles, we deduced the specific conductivity of the contractile filaments kf and their volume fraction rho (kf = 2.78 X 10(-3) omega-1cm-1, and rho = 0.48). The experimental technique permits an estimate of the specific myoplasmic conductivity in vivo (6.27 X 10(-3) omega-1cm-1). Finally, a decrease in the pH of the external solution from 10.1 to 4.0 lowered the myoplasmic conductivity by 16%. This may be considered as indirect evidence that the conductivity of the contractile filaments is associated with the protein counter-ions, since Hinke et al. (1973. Ann. N.Y. Acad. Sci. 204, 274-296.) reported evidence that a lowering of pH decreases the number of counter-ions.
在置于圆柱形腔室中的藤壶（Balanus nubilus）单根肌纤维上测量肌质阻抗，该腔室用于限制体积并防止肌质水合。在腔室的两端，肌质与电解质溶液直接接触。当与外部介质达到平衡时，在10℃下用阻抗电桥在1000Hz测量肌质阻抗。结果表明，肌纤维的肌质阻抗主要是电阻性的。将肌质视为小导电颗粒的悬浮液，我们推导出收缩细丝的电导率kf及其体积分数rho（kf = 2.78×10^(-3)Ω^(-1)cm^(-1)，rho = 0.48）。该实验技术允许估计体内肌质的比电导率（6.27×10^(-3)Ω^(-1)cm^(-1)）。最后，外部溶液的pH从10.1降至4.0使肌质电导率降低了16%。这可被视为收缩细丝电导率与蛋白质抗衡离子相关的间接证据，因为Hinke等人（1973年。《纽约科学院学报》204, 274 - 296）报道了pH降低会减少抗衡离子数量的证据。
