Bayer E A, Rivnay B, Skutelsky E
Biochim Biophys Acta. 1979 Feb 2;550(3):464-73. doi: 10.1016/0005-2736(79)90149-4.
An efficient method for labeling and visualizing phospholipids at the ultrastructural level is described. Biotin was coupled to the amines of appropriate phospholipids via the N-hydroxysuccinimide ester. The biotinylated lipid could be specifically labeled by ferritin-avidin conjugates and detected by transmission electron microscopy. The lipid derivatives were analyzed and evaluated in terms of their resemblance to the original lipid. Although differing in some aspects from the parent lipid molecules, the biotinyl derivatives still retain the basic characteristics of lipids vis-a-vis their orientation and position in the membrane bilayer. The latter property renders the biotinylated lipid qualitatively suitable for tracing the fate of the lipid component(s) of liposomes during their interaction with biological membranes of various cell types. Using this system, we propose that the extent and pattern of the liposome-cell interaction depends, at least in part, on the cell type employed. This observation may be due to intrinsic variations in cell surface structure and properties relative to those of the liposome.
本文描述了一种在超微结构水平上标记和可视化磷脂的有效方法。通过N-羟基琥珀酰亚胺酯将生物素与适当磷脂的胺基偶联。生物素化的脂质可以被铁蛋白-抗生物素蛋白缀合物特异性标记，并通过透射电子显微镜检测。对脂质衍生物进行了分析，并根据它们与原始脂质的相似性进行了评估。尽管生物素基衍生物在某些方面与母体脂质分子不同，但它们在膜双层中的取向和位置方面仍保留了脂质的基本特征。后一特性使生物素化脂质在定性上适合追踪脂质体的脂质成分在与各种细胞类型的生物膜相互作用过程中的命运。使用该系统，我们提出脂质体与细胞相互作用的程度和模式至少部分取决于所使用的细胞类型。这一观察结果可能是由于细胞表面结构和性质相对于脂质体的固有差异所致。
