Benjamin W B, Singer I
Science. 1974 Oct 18;186(4160):269-72. doi: 10.1126/science.186.4160.269.
Simultaneous electrophysiological and biochemical experiments demonstrated a specific aldosterone-induced protein in paired urinary hemibladders isolated from the toad Bufo marinus. Whenever aldosterone stinlmulated short-circuit current, aldosterone specifically increased [(35)S] methionine incorporation into a low-molecular-weight protein (about 12,000). Comparative studies with dexamethasone and insulin and inhibitory studies with spironolactone and actinomycin D suggest mineralocorticoid specificity.
同步进行的电生理和生化实验表明，从海蟾蜍（Bufo marinus）分离出的成对膀胱中存在一种由醛固酮特异性诱导产生的蛋白质。每当醛固酮刺激短路电流时，醛固酮会特异性地增加[（35）S]甲硫氨酸掺入一种低分子量蛋白质（约12,000）中的量。用地塞米松和胰岛素进行的对比研究以及用螺内酯和放线菌素D进行的抑制性研究表明存在盐皮质激素特异性。
