Crabbé J
Pflugers Arch. 1980 Jan;383(2):151-8. doi: 10.1007/BF00581876.
The reversible inhibition of transepithelial sodium transport achieved with amiloride (and triamterene) was evaluated in amphibian preparations stimulated with aldosterone so as to provide further information regarding a possible influence of this hormone on the apical border of target cells. When aldosterone secretion was enhanced by withdrawal of sodium from toad (Bufo marinus) habitat, sensitivity of abdominal skin to amiloride decreased; the same occurred in skin and bladder preparations incubated with aldosterone for several hours. Amiloride proved a less efficient blocker of sodium transport by toad skin exposed to vasopressin and to ouabain; both substances are capable or raising cell sodium content. It is therefore proposed that the decrease in sensitivity to amiloride of amphibian epithelial treated with aldosterone results from an increase in target cell sodium, itself due to a hormone-induced increas in sodium conductance at the apical cell border. Glucose, which enhanced markedly the rate of sodium transport in preparations treated with aldosterone for several hours, failed to decrease any further the response to amiloride; this is taken as an argument for an additional (? secondary) influence of aldosterone on the cell's metabolic machinery connected with the operation of the sodium 'pump'.
在醛固酮刺激的两栖类标本中评估了用氨氯吡脒（和氨苯蝶啶）实现的跨上皮钠转运的可逆抑制作用，以便提供关于该激素对靶细胞顶端边界可能影响的更多信息。当通过从蟾蜍（海蟾蜍）栖息地去除钠来增强醛固酮分泌时，腹部皮肤对氨氯吡脒的敏感性降低；在用醛固酮孵育数小时的皮肤和膀胱标本中也出现同样情况。氨氯吡脒被证明是蟾蜍皮肤暴露于血管加压素和哇巴因时钠转运的效率较低的阻断剂；这两种物质都能够提高细胞钠含量。因此有人提出，用醛固酮处理的两栖类上皮对氨氯吡脒敏感性的降低是由于靶细胞钠增加，这本身是由于激素诱导的顶端细胞边界钠电导增加所致。葡萄糖显著提高了用醛固酮处理数小时的标本中的钠转运速率，但未能进一步降低对氨氯吡脒的反应；这被视为醛固酮对与钠“泵”运作相关的细胞代谢机制有额外（？次要）影响的一个论据。
