Gailani S, McLimans W F, Mundy G R, Nussbaum A, Roholt O, Zeigel R
Cancer Res. 1976 Apr;36(4):1299-304.
Bone marrow biopsy specimens from patients with myeloma were cultured in either 1 of 2 thin-film culture systems, a controlled environment steady state system or a rocker tube configuration of the system, for periods up to 42 days. Both functional and morphological characteristics of the myeloma cells were well-maintained in these systems. Cytocentrifuge preparations of the culture media disclosed hematopoietic cells that included from 5% to almost 100% plasma cells. Histological examination of the cultured specimens disclosed infiltration of the marrow with myeloma cells. Myeloma proteins were released at a steady rate throughout the period of culture after the 1st 4 days. Bone-resorbing activity was demonstrated in the culture media in 7 of 9 myeloma culture media and was well maintained, particularly during the 1st week of culture. This activity was associated with severe osteolytic lesions in the donor patient and marked infiltration of the cultured specimen by myeloma cells. The potential use of these organ culture systems for the further definitive identification of the factor responsible for bone destruction in myeloma is discussed.
将骨髓瘤患者的骨髓活检标本在两种薄膜培养系统中的一种中培养，即受控环境稳态系统或该系统的摇管配置，培养时间长达42天。在这些系统中，骨髓瘤细胞的功能和形态特征均得到良好维持。培养基的细胞离心涂片显示造血细胞，其中包括5%至几乎100%的浆细胞。对培养标本的组织学检查显示骨髓瘤细胞浸润骨髓。在最初4天后的整个培养期间，骨髓瘤蛋白以稳定的速率释放。在9份骨髓瘤培养基中的7份中，培养基显示出骨吸收活性，且该活性得到良好维持，尤其是在培养的第一周。这种活性与供体患者的严重溶骨性病变以及培养标本中骨髓瘤细胞的明显浸润有关。讨论了这些器官培养系统在进一步明确鉴定导致骨髓瘤骨破坏的因素方面的潜在用途。
