Hill M W, Squier C A
J Anat. 1979 Jan;128(Pt 1):169-78.
The intercellular permeability barrier of neonatal rat palatal mucosa maintained in organ culture for periods up to 24 days was studied ultrastructurally using the tracers horseradish peroxidase and lanthanum nitrate. At all time intervals examined the limit of penetration of the tracers corresponded to the level at which the membrane-coating granules were being discharged. However, in the cultured mucosa, extrusion of granules occurred closer to the granular cell-keratin junction after 6 and 12 days in vitro than at other time intervals. This probably is a reflexion of the higher rate of tissue turnover at these times. It is concluded that a permeability barrier comparable with that described in vivo is produced by the epithelium during maintenance inorgan culture and is further evidence of the functional integrity of the tissue in vitro.
利用辣根过氧化物酶和硝酸镧示踪剂，对在器官培养中维持长达24天的新生大鼠腭黏膜的细胞间渗透屏障进行了超微结构研究。在所有检查的时间间隔内，示踪剂的渗透极限与膜包被颗粒排出的水平相对应。然而，在培养的黏膜中，体外培养6天和12天后，颗粒的挤出比其他时间间隔更靠近颗粒细胞 - 角质连接。这可能反映了这些时期组织更新率较高。结论是，上皮细胞在器官培养维持过程中产生了与体内描述的相当的渗透屏障，这进一步证明了该组织在体外的功能完整性。
