由大鼠腹水肝癌AH 7974细胞的多聚腺苷酸（poly(A)）+和多聚腺苷酸（poly(A)）-信使核糖核酸（mRNA）体外合成的多肽。

Polypeptides synthesized in vitro by poly(A)+ and poly(A)- mRNAs of rat ascites hepatoma AH 7974 cells.

作者信息

Sakiyama S

出版信息

J Biochem. 1979 Feb;85(2):609-13. doi: 10.1093/oxfordjournals.jbchem.a132370.

DOI:10.1093/oxfordjournals.jbchem.a132370

Abstract

Polysomal RNA of rat ascites hepatoma AH 7974 cells was fractionated into poly(A)+ and poly(A)- mRNAs and these RNAs were translated into polypeptides in a protein-synthesizing system derived from wheat germ. The analysis of polypeptides synthesized in vitro by two-dimensional gel electrophoresis revealed that there are three classes of polypeptides. The first group can be synthesized equally by both poly(A)+ and poly(A)- mRNAs; the second and third groups are synthesized predominantly by poly(A)+ and poly(A)- mRNAs, respectively. These results suggest that the three classes of polypeptides can be characterized by the presence or absence of poly(A) tails of the corresponding mRNAs.

摘要

大鼠腹水肝癌AH 7974细胞的多聚核糖体RNA被分离为多聚腺苷酸尾（poly(A)+）和非多聚腺苷酸尾（poly(A)-）的信使核糖核酸（mRNA），这些RNA在源自小麦胚芽的蛋白质合成系统中被翻译成多肽。通过双向凝胶电泳对体外合成的多肽进行分析，结果显示存在三类多肽。第一类多肽可由poly(A)+和poly(A)- mRNA同等合成；第二类和第三类多肽分别主要由poly(A)+和poly(A)- mRNA合成。这些结果表明，这三类多肽可通过相应mRNA的poly(A)尾的有无来表征。