Hamburger A W, Kim M B, Salmon S E
J Cell Physiol. 1979 Feb;98(2):371-6. doi: 10.1002/jcp.1040980213.
Freshly explanted human myeloma cells formed colonies of monoclonal plasma cells in soft agar in the presence of medium conditioned by the adherent spleen cells of mineral oil-primed BALB/c mice. The medium showed peak activity at a dilution of 1:4. 2-mercaptoethanol or monothioglycerol was necessary for colony formation. Other thiols tested were ineffective in promoting colony growth. Colony-forming cells adhered to nylon wool, but not glass beads or plastic dishes. The presence of E-rosetting cells was not required for myeloma colony formation. Antibody prepared against a human myeloma cell line, RPMI 8226, reduced colony formation. These studies demonstrate the usefulness of this bioassay for determining functional properties of the myeloma colony-forming cell.
新分离出的人骨髓瘤细胞在由矿物油致敏的BALB/c小鼠的贴壁脾细胞条件培养液存在的情况下，能在软琼脂中形成单克隆浆细胞集落。该培养液在1:4稀释时显示出峰值活性。2-巯基乙醇或单硫甘油是集落形成所必需的。所测试的其他硫醇在促进集落生长方面无效。集落形成细胞能黏附于尼龙棉，但不黏附于玻璃珠或塑料培养皿。骨髓瘤集落形成不需要E-玫瑰花结形成细胞的存在。针对人骨髓瘤细胞系RPMI 8226制备的抗体可减少集落形成。这些研究证明了这种生物测定法在确定骨髓瘤集落形成细胞功能特性方面的实用性。
