尿11-脱氧-17-氧代类固醇羧酸酯的气液色谱法。以正丁酸酯形式测定。
The gas-liquid chromatography of carboxylic acid esters of the urinary 11-deoxy-17-oxo steroids. Determination as n-butyrates.
作者信息
Sadler P A, Kellie A E
出版信息
Biochem J. 1967 Jun;103(3):768-72. doi: 10.1042/bj1030768.
Abstract
- The gas-liquid-chromatographic separations of the acetate, propionate, n-butyrate, isobutyrate and n-valerate esters of androsterone, aetiocholanolone and dehydroepiandrosterone were studied on a 1% neopentyl glycol sebacate column. The n-butyrate, isobutyrate and n-valerate esters were well resolved. 2. The three steroids derived from hydrolysed urinary 17-oxo steroid conjugate extracts were analysed by gas-liquid chromatography after conversion into their n-butyrate esters. The results were compared with independent determinations involving chromatography on alumina.
摘要
- 在1%新戊二醇癸二酸酯柱上研究了雄甾酮、本胆烷醇酮和脱氢表雄酮的乙酸酯、丙酸酯、正丁酸酯、异丁酸酯和正戊酸酯的气液色谱分离。正丁酸酯、异丁酸酯和正戊酸酯得到了很好的分离。2. 水解后的尿17-氧代甾体共轭提取物衍生出的三种甾体，在转化为正丁酸酯后通过气液色谱进行分析。将结果与涉及氧化铝色谱的独立测定结果进行了比较。
相似文献
1
The gas-liquid chromatography of carboxylic acid esters of the urinary 11-deoxy-17-oxo steroids. Determination as n-butyrates.
Biochem J. 1967 Jun;103(3):768-72. doi: 10.1042/bj1030768.
2
Routine assay of 11-deoxy-17-ketosteroids in human urinary gas-liquid chromatography with a standardized column.
Am J Clin Pathol. 1967 Sep;48(3):295-300. doi: 10.1093/ajcp/48.3.295.
3
[Determination of the principal 17-ketosteroids in the urine by vapor phase chromatography].
Ann Biol Clin (Paris). 1968 Jul-Sep;26(7):949-55.
4
[Study of the dynamics of female urinary 11-deoxy-17-ketosteroid fractions studies by gas-liquid chromatography].
Nihon Sanka Fujinka Gakkai Zasshi. 1968 Dec;20(12):1599-608.
5
[The urinary 11-desoxy-17-ketosteroids (androsterone, etiocholanolone, dehydroisoandrosterone) in childhood. Gas chromatographic study].
Clin Pediatr (Bologna). 1968 Oct;50(10):654-64.
6
Rapid quantitative gas-chromatographic fractionation of the major urinary 17-oxosteroids and 17-oxogenic steroids.
Clin Chim Acta. 1972 Sep;40(2):421-30. doi: 10.1016/0009-8981(72)90354-3.
7
8
The quantitation of major 17-ketosteroid fractions by gas-liquid chromatography.
Clin Chem. 1966 Jul;12(7):399-405.
9
[Gas chromatographic determination of urinary 17-ketosteroids].
Boll Soc Ital Biol Sper. 1976 Oct 30;52(20):1736-9.
10
Chloromethyldimethylsilyl ethers in the routine assay of urinary 11-deoxy-17-oxosteroids by gas-liquid chromatography.
J Endocrinol. 1968 Jun;41(2):203-11. doi: 10.1677/joe.0.0410203.
本文引用的文献
1
Colorimetric determination of substances containing the grouping -CH(2).CO- in urine extracts as an indication of androgen content.
Biochem J. 1938 Aug;32(8):1312-31. doi: 10.1042/bj0321312.
2
Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues.
Biochem J. 1952 Jan;50(3):370-8. doi: 10.1042/bj0500370.
3
Rm values in the gas-liquid chromatography of steroids.
Nature. 1962 Jun 2;194:833-5. doi: 10.1038/194833a0.
4
DETERMINATION OF ANDROSTERONE, ETIOCHOLANOLONE AND DEHYDROEPIANDROSTERONE IN URINE BY GAS-LIQUID CHROMATOGRAPHY.
Steroids. 1965 May;6:687-97. doi: 10.1016/0039-128x(65)90161-3.
5
Separation and estimation of the principal human urinary 17-ketosteroids as trimethylsilyl ethers.
Anal Biochem. 1962 Sep;4:191-7. doi: 10.1016/0003-2697(62)90001-5.
6
Separation of urinary 17-ketosteroids by gas chromatography.
Anal Biochem. 1961 Apr;2:182-7. doi: 10.1016/0003-2697(61)90070-7.
7
Hydrolysis of ketosteroid hydrogen sulfates by solvolysis procedures.
J Biol Chem. 1958 Aug;233(2):331-5.
8
The analysis of urinary 17-oxo steroids by gradient elution.
Biochem J. 1957 Jun;66(2):196-206. doi: 10.1042/bj0660196.
Zotero 插件