Wick M M, Byers L, Ratliff J
J Invest Dermatol. 1979 Feb;72(2):67-9. doi: 10.1111/1523-1747.ep12530286.
The growth inhibitory effect of 6-hydroxydopa, a cytotoxic analog of L-dopa, was studied in melanotic and amelanotic Cloudman melanoma, mouse fibroblast L929 and Chinese hamster ovary cells. A marked sensitivity of pigmented cells to 6-hydroxydopa was observed with a 10-fold increase in sensitivity of pigmented cells. Sensitivity correlated with the capacity of cells to incorporate radiolabeled exogenous L-dopa. The drug affected primarily DNA and RNA synthesis with greater inhibition observed in pigmented cells than the nonpigmented control cells. The mechanism of action may involve interaction with the melanocyte specific enzyme, tyrosinase, as a false substrate.
研究了L-多巴的细胞毒性类似物6-羟基多巴对黑素性和无黑素性Cloudman黑色素瘤、小鼠成纤维细胞L929和中国仓鼠卵巢细胞的生长抑制作用。观察到色素细胞对6-羟基多巴具有明显的敏感性，色素细胞的敏感性增加了10倍。敏感性与细胞摄取放射性标记外源性L-多巴的能力相关。该药物主要影响DNA和RNA合成，色素细胞中的抑制作用比无色素对照细胞更强。其作用机制可能涉及作为假底物与黑素细胞特异性酶酪氨酸酶相互作用。
