Kondo S, Aso K, Namba M
J Invest Dermatol. 1979 Feb;72(2):85-7. doi: 10.1111/1523-1747.ep12530300.
Human epidermal cells were cultured with lethally irradiated 3T3 cells and grew as large colonies both on plastic and millipore filters. Subcultures were possible. The grown colonies were studied morphologically and histologically. The whole intact crossview of epidermal colonies in paraffin sections was obtained from the cell culture on millipore filters. The epidermal cells derived from younger persons had higher efficiency of colony formation than those from older ones. The membrane filter was shown to be good substratum for epidermal cell growth as well as plastic.
将人表皮细胞与经致死剂量照射的3T3细胞共同培养，这些细胞在塑料和微孔滤膜上均能形成大的集落，并且可以进行传代培养。对生长的集落进行了形态学和组织学研究。通过在微孔滤膜上进行细胞培养，在石蜡切片中获得了表皮集落完整的全剖视图。较年轻个体来源的表皮细胞比年老者来源的细胞具有更高的集落形成效率。结果表明，膜滤器与塑料一样，是表皮细胞生长的良好基质。
