Stone C L, Mahaffey K R, Fox M R
J Environ Pathol Toxicol. 1979 Jan-Feb;2(3):767-79.
A rapid bioassay of lead was established in young japanese quail. Each group of 10 day-old birds received deionized water and a purified diet ad libitum for 2 wk. The diet contained 0.2 microgram lead/g. Lead acetate was added to give 14.8, 34.4, 51.2, 74.4, 234, 563, or 1223 microgram total lead/g by analysis. The duodenum, kidneys, liver and tibias were assayed for lead. Consumption of a diet containing either 563 or 1223 ppm lead caused a decrease (P less than or equal to 0.05) in body weight after 1 wk and an increase in free erythrocyte protoporphyrin after 2wk. Inhibition of red blood cell delta-aminolevulinic acid dehydratase (RBC-ALAD) activity occurred in birds consuming as little as 14.8 ppm lead. Packed cell volumes and hemoglobin concentrations were not affected by the dietary treatments. Lead concentrations in tissues from birds fed the lowest level of added lead were greater than those found in the corresponding tissues of control birds. The concentration of lead in the tibia showed the most distinguishable group means and the most nearly linear response; the highest slope was between 14.8 and 110 ppm dietary lead, using log-log transformations. The rapid bioassay is suitable for investigating high lead foods.
在日本鹌鹑幼雏中建立了一种快速铅生物测定法。每组10日龄的雏鸟自由饮用去离子水并随意采食纯化日粮，持续2周。日粮含铅量为0.2微克/克。通过分析添加醋酸铅，使日粮总铅含量达到14.8、34.4、51.2、74.4、234、563或1223微克/克。对十二指肠、肾脏、肝脏和胫骨进行铅含量测定。采食含563或1223 ppm铅日粮1周后体重下降（P≤0.05），2周后游离红细胞原卟啉增加。采食低至14.8 ppm铅日粮的鸟类红细胞δ-氨基乙酰丙酸脱水酶（RBC-ALAD）活性受到抑制。红细胞压积和血红蛋白浓度不受日粮处理的影响。采食添加铅水平最低日粮的鸟类组织中的铅浓度高于对照鸟类相应组织中的铅浓度。胫骨中的铅浓度显示出最明显的组间均值差异和最接近线性的反应；采用对数-对数转换，最高斜率出现在日粮铅含量为14.8至110 ppm之间。这种快速生物测定法适用于研究高铅食物。
