Cooper T B, Kelly R G
J Pharm Sci. 1979 Feb;68(2):216-9. doi: 10.1002/jps.2600680224.
A GLC analysis is presented for loxapine, amoxapine, and their major metabolites in serum and urine. Electron-capture detection is employed for serum analysis, and flame ionization is used for urine analysis. The procedure includes trifluoroacetylation of secondary amine functions, followed by trimethylsilylation of phenolic groups after ethyl acetate extraction of the sample. Urine requires prior enzymatic hydrolysis of conjugates. Data indicating the utility of the procedure in hospitalized patients and normal volunteers are presented.
本文介绍了对血清和尿液中洛沙平、阿莫沙平及其主要代谢物的气相色谱（GLC）分析。血清分析采用电子捕获检测，尿液分析则使用火焰离子化检测。该方法包括仲胺官能团的三氟乙酰化，然后在样品用乙酸乙酯萃取后对酚羟基进行三甲基硅烷基化。尿液需要先进行结合物的酶促水解。文中给出了该方法在住院患者和正常志愿者中应用的相关数据。
