Quantitative determination of hexylresorcinol in commercial antiseptic solution by high-pressure liquid chromatography.

High-pressure liquid chromatography was used with a 5-micron silica gel column to quantitate hexylresorcinol in a commercial antiseptic solution following extraction with methylene chloride. This method shows linearity to at least 0.025% hexylresorcinol. A mobile phase consisting of 63% heptane, 34% chloroform, and 5% methanol was used with a UV detector (254 nm) and a flow of 3 ml/min. No interfering substances were observed.