Smallwood A W, Ranieri T L, Satzger R D
U.S. Food and Drug Administration, National Forensic Chemistry Center, Cincinnati, OH 45202, USA.
J AOAC Int. 1998 Mar-Apr;81(2):488-91.
A method is described for determining 4-hexylresorcinol in crab meat. 4-Hexylresorcinol is used to prevent melanosis in shrimp, and the same use has been proposed for crab meat. Because 4-hexylresorcinol may be added illegally to crab meat as a preservative, consumer protection requires that residues of the compound be monitored in crab meat. 4-Hexylresorcinol is extracted from crab meat with acetonitrile. After dilution with water, the extract is passed through a C18 solid-phase extraction column and 4-hexylresorcinol is eluted from the column with ethanol. The compound is determined by reversed-phase liquid chromatography with diode array detection at 206 nm. Limit of quantitation is 1.0 microgram/g. Mean recovery in the range 1-20 micrograms/g is 89%, with a relative standard deviation of 6.3.
描述了一种测定蟹肉中4-己基间苯二酚的方法。4-己基间苯二酚用于防止虾的黑变病，并且有人提议将其用于蟹肉。由于4-己基间苯二酚可能作为防腐剂被非法添加到蟹肉中，为保护消费者，需要监测蟹肉中该化合物的残留量。用乙腈从蟹肉中提取4-己基间苯二酚。用水稀释后，提取物通过C18固相萃取柱，然后用乙醇从柱上洗脱4-己基间苯二酚。该化合物通过反相液相色谱法在206nm处用二极管阵列检测进行测定。定量限为1.0微克/克。在1-20微克/克范围内的平均回收率为89%，相对标准偏差为6.3。
