Kanagawa H, Basrur P K
Can J Comp Med. 1968 Oct;32(4):583-6.
The clinical application and reliability of the leukocyte culture method for the diagnosis of freemartinism were examined and the length of time that blood samples could be held at room temperature and in the refrigerator prior to culturing, was investigated. The chromosome findings by the leukocyte culture method in 14 freemartins and 9 non-freemartin females belonging to heterosexual twins or triplets revealed that XX-XY cell chimerism exists only in the former, whereas the latter were exclusively of normal female complement. The mitotic index in bovine blood after preservation for varying periods was studied on samples from two animals. Blood samples from these two animals stored at 5 degrees C for 6 hours in a refrigerator showed the mitotic index to be 3.8 and 5.3 per cent which gradually decreased in samples stored for longer than 12 hours. After 72 hours, a very rapid decrease in mitotic index occurred in both cases, reaching zero in samples stored for 96 and 108 hours. Samples kept at room temperature followed a similar pattern as under refrigeration but with slightly lower values throughout.
对用于诊断自由马丁症的白细胞培养方法的临床应用和可靠性进行了研究，并调查了血液样本在培养前可在室温及冰箱中保存的时间长度。通过白细胞培养方法对14例自由马丁症雌性和9例属于异性双胞胎或三胞胎的非自由马丁症雌性进行染色体检查，结果显示XX-XY细胞嵌合体仅存在于前者中，而后者均为正常的女性染色体组成。对来自两只动物的样本研究了保存不同时间段后牛血液中的有丝分裂指数。这两只动物的血液样本在冰箱中5℃保存6小时后，有丝分裂指数分别为3.8%和5.3%，在保存超过12小时的样本中该指数逐渐下降。72小时后，两种情况下有丝分裂指数均急剧下降，在保存96小时和108小时的样本中降至零。保存在室温下的样本遵循与冷藏类似的模式，但始终数值略低。
