Vermes I, Várszegi M, Tóth E K, Telegdy G
Arch Androl. 1979;3(2):127-33. doi: 10.3109/01485017908985059.
The effects of different neurotransmitters were tested in vitro on a hypothalamic tissue, collagenase-digested isolated anterior pituitary and Leydig cell suspension system by measuring the testosterone production of the Leydig cells. Neurotransmitters were used in concentrations of 0.25, 1.0, 2.5, 5.0, and 10.0 micrograms/ml incubation medium. Dopamine in doses of 1.0, 2.5, and 5.0 micrograms/ml increased the hypothalamic tissue-induced pituitary-testis activation, while it had no direct effect on pituitary and Leydig cells. Noradrenaline in the concentration range 2.5--10.0 micrograms/ml decreased the luteinizing-hormone-releasing-hormone (LHRH) sensitivity of the pituitary cells. 5.0 and 10.0 micrograms/ml 5-hydroxytryptamine decreased the testosterone production and the hCG sensitivity of the isolated Leydig cells. Carbamylcholine and pilocarpine had no action on the in vitro system at the different levels studied.
通过测量睾丸间质细胞的睾酮生成量，在体外对下丘脑组织、胶原酶消化的离体垂体前叶和睾丸间质细胞悬浮系统测试了不同神经递质的作用。神经递质在孵育培养基中的浓度分别为0.25、1.0、2.5、5.0和10.0微克/毫升。剂量为1.0、2.5和5.0微克/毫升的多巴胺增强了下丘脑组织诱导的垂体-睾丸激活，而对垂体和睾丸间质细胞无直接作用。浓度范围在2.5至10.0微克/毫升的去甲肾上腺素降低了垂体细胞对促黄体生成素释放激素（LHRH）的敏感性。5.0和10.0微克/毫升的5-羟色胺降低了离体睾丸间质细胞的睾酮生成量和对人绒毛膜促性腺激素（hCG）的敏感性。在所研究的不同水平上，氨甲酰胆碱和毛果芸香碱对体外系统无作用。
