Nazina T N, Rozanova E P, Kalininskaia T A
Mikrobiologiia. 1979 Jan-Feb;48(1):133-6.
The activity of nitrogen fixation by the museum strains of sulfate reducing bacteria isolated from oil deposits was studied using the acetylene technique. The mesophilic sulpfate reducing bacteria belonging to the species Desulfovibrio africanus 2372 and D. baculatus X were found to have a high nitrogenase activity. D desulfuricans subsp. aestuarii 2198 reduced acetylene at a low rate. The thermophilic sulfate reducing cultures of D. thermophilus 7, Desulfotomaculum nigrificans 781 and Dm. nigrificans subsp. salinus 435 produced only small quantities of ethylene. Apparently, nitrogen fixation by sulfate reducing bacteria can be considerable in oil strata whose temperature does not exceed 35--40 degrees C.
利用乙炔技术研究了从油藏中分离出的博物馆硫酸盐还原菌菌株的固氮活性。发现属于非洲脱硫弧菌2372和杆状脱硫弧菌X的嗜温硫酸盐还原菌具有较高的固氮酶活性。盐沼脱硫脱硫弧菌亚种2198以低速率还原乙炔。嗜热硫酸盐还原培养物嗜热脱硫弧菌7、黑色脱硫肠状菌781和黑色脱硫肠状菌盐沼亚种435仅产生少量乙烯。显然，在温度不超过35 - 40摄氏度的油层中，硫酸盐还原菌的固氮作用可能相当可观。
