Schuytema E C, Glenn H L, Doughty C C
J Bacteriol. 1969 Jun;98(3):920-3. doi: 10.1128/jb.98.3.920-923.1969.
Lytic enzymes were isolated from 14 strains of phage-infected Staphylococcus aureus. Cell walls were prepared from the same uninfected strains of bacteria. Comparison of the lytic rates was made for each enzyme, with each of the cell walls as substrate. Differences in the rate of substrate utilization of the various cell wall types exceeded 10-fold. Cell walls from strains 42E, 29, and 77 were the best substrates, whereas cell walls from strains 3C, 80, and 187 were the poorest substrates. The cell wall amino acid composition is discussed as related to lytic enzyme specificity. A possible explanation of phage typing of staphylococcal cells, based on enzyme activity and cell wall composition, is presented.
从14株噬菌体感染的金黄色葡萄球菌中分离出裂解酶。从相同的未感染细菌菌株制备细胞壁。以每种细胞壁为底物，对每种酶的裂解速率进行了比较。各种细胞壁类型的底物利用速率差异超过10倍。42E、29和77菌株的细胞壁是最佳底物，而3C、80和187菌株的细胞壁是最差底物。讨论了与裂解酶特异性相关的细胞壁氨基酸组成。基于酶活性和细胞壁组成，提出了对葡萄球菌细胞进行噬菌体分型的一种可能解释。
