Kohler E M, Cross R F
Can J Comp Med. 1969 Jul;33(3):173-7.
Nine gnotobiotic pigs derived from one gilt were fed bacteria-free filtrates prepared from: 1) cultures of an enteropathogenic strain of Escherichia coli 09:K.:NM (Strain 340), 2) cultures of a nonenteropathogenic strain of E. coli 08.K..H16 (Strain CDC-1466-56), and 3) uninoculated culture medium. Diarrhea was observed initially two to four hours after feeding the filtrate prepared from the enteropathogenic E. coli. The duration of diarrhea was five to ten hours. No diarrhea was observed after feeding filtrate prepared from uninoculated medium or cultures of nonenteropathogenic E. coli. The pH values of the feces increased with the onset of diarrhea and decreased to normal after diarrhea stopped. No histopathological lesions were found.
从一头母猪繁育出的9只无菌猪被喂食了由以下物质制备的无菌滤液：1）致病性大肠杆菌09:K.:NM菌株（340菌株）的培养物；2）非致病性大肠杆菌08.K..H16菌株（CDC-1466-56菌株）的培养物；3）未接种的培养基。喂食致病性大肠杆菌制备的滤液后最初两到四小时观察到腹泻。腹泻持续时间为五到十小时。喂食未接种培养基或非致病性大肠杆菌培养物制备的滤液后未观察到腹泻。粪便的pH值随着腹泻的开始而升高，并在腹泻停止后降至正常。未发现组织病理学损伤。
