Morgante O, Shemanchuk J A, Windsor R
Can J Comp Med. 1969 Jul;33(3):227-30.
Sera from indicator chicken flocks situated in southern Alberta near Lethbridge were tested by the hemagglutination-inhibition (HI) technique for antibodies to Western, Eastern and St. Louis Encephalitis viruses during the summer and early fall of the four years 1964 to 1967.One chicken in 1964, 90 in 1965 and five each in 1966 and 1967 were positive to Western encephalomyelitis (WE) virus by hemagglutination-inhibition tests. All of the positive sera were confirmed by neutralization test (NT) in infant mice. No antibodies to the Eastern and St. Louis viruses were detected. Infection with WE virus was detected in each of the four years, indicating that WE virus is endemic to southern Alberta with a marked seasonal incidence occurring between the second week in August and the third week in September. An improved technique for filtering sera is described.
1964年至1967年的夏季和初秋期间，采用血凝抑制（HI）技术对位于莱斯布里奇附近的艾伯塔省南部指示鸡群的血清进行检测，以检测针对西部、东部和圣路易斯脑炎病毒的抗体。1964年有1只鸡、1965年有90只鸡以及1966年和1967年各有5只鸡通过血凝抑制试验对西部脑脊髓炎（WE）病毒呈阳性反应。所有阳性血清均在新生小鼠中通过中和试验（NT）得到证实。未检测到针对东部和圣路易斯病毒的抗体。在这四年中的每一年都检测到了WE病毒感染，这表明WE病毒在艾伯塔省南部呈地方性流行，在8月的第二周和9月的第三周之间出现明显的季节性发病情况。本文描述了一种改进的血清过滤技术。
