植物血凝素刺激的人淋巴细胞中的胞苷5'-二磷酸还原酶活性
Cytidine 5'-diphosphate reductase activity in phytohemagglutinin stimulated human lymphocytes.
作者信息
Tyrsted G, Gamulin V
出版信息
Nucleic Acids Res. 1979 Jan;6(1):305-19. doi: 10.1093/nar/6.1.305.
Abstract
The optimal conditions and the effect of deoxyribonucleoside triphosphates were determined for CDP reductase activity in PHA-stimulated lymphocytes. The enzymatic reaction showed an absolute requirement for ATP. In the absence of ATP, only dATP showed a minor stimulation of the reduction of CDP to dCDP. During transformation the CDP reductase activity reached a maximum at the same time as the four deoxyribonucleoside triphosphate pools, corresponding to mid S-phase at about 50 h after PHA addition. The DNA polymerase activity reached a maximum at 57 h.
摘要
确定了PHA刺激的淋巴细胞中CDP还原酶活性的最佳条件以及三磷酸脱氧核糖核苷的作用。酶促反应显示对ATP有绝对需求。在没有ATP的情况下，只有dATP对CDP还原为dCDP有轻微刺激作用。在转化过程中，CDP还原酶活性与四个三磷酸脱氧核糖核苷池同时达到最大值，对应于添加PHA后约50小时的S期中期。DNA聚合酶活性在57小时达到最大值。
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