Mkrtumova N A, Lobanova A M
Probl Endokrinol (Mosk). 1979 Mar-Apr;25(2):57-61.
The authors studied insulin and proinsulin degradation with homogenates of various rat tissues under normal conditions and in alloxan diabetes. The radioimmunological method was used for insulin determination by the decline of immunoreactive insulin and proinsulin from the reaction medium. Homogenates of various normal rat tissues are distributed by their capacity to destroy insulin and proinsulin in the order of decline as follows: the liver, kidneys, muscles, and the epididymal fat. In insulin equimolar quantities proinsulin was destroyed much less than insulin under the same experimental conditions. Alloxan diabetes led to reduction of the capacity of all the tissues homogenates to destroy both insulin and proinsulin. Reduction of degradation was the most pronounced when the muscle and adipose tissue homogenates were used.
作者研究了在正常条件下以及四氧嘧啶糖尿病状态下，各种大鼠组织匀浆对胰岛素和胰岛素原的降解情况。采用放射免疫法，通过反应介质中免疫反应性胰岛素和胰岛素原的减少来测定胰岛素。各种正常大鼠组织匀浆按照破坏胰岛素和胰岛素原能力下降的顺序排列如下：肝脏、肾脏、肌肉和附睾脂肪。在相同实验条件下，等摩尔量的胰岛素原比胰岛素被破坏的程度要小得多。四氧嘧啶糖尿病导致所有组织匀浆破坏胰岛素和胰岛素原的能力下降。当使用肌肉和脂肪组织匀浆时，降解减少最为明显。
