兔耳软骨硫酸软骨素的代谢异质性
The metabolic heterogeneity of rabbit ear cartilage chondroitin sulphate.
作者信息
Bentley J P, Rokosová B
出版信息
Biochem J. 1970 Feb;116(3):329-36. doi: 10.1042/bj1160329.
Abstract
The only glycosaminoglycans that can be isolated from the ear cartilage of 2-month-old rabbits are chondroitin 4-sulphate and chondroitin 6-sulphate. These chondroitin sulphates exhibit molecular-weight polydispersity when isolated from tissue by papain digestion. The chondroitin sulphate is metabolically heterogeneous in that radioactive precursors [(14)C]glucose or [(35)S]sulphate are preferentially incorporated into the higher-molecular-weight polymers both in vivo and in vitro. No transfer of radioactivity from the high-molecular-weight chondroitin sulphate to the low-molecular-weight chondroitin sulphate was seen during 15 days in vivo. It is suggested that there are at least two pools of proteoglycan in the tissue. One of these pools is metabolically active whereas the other is not.
摘要
从2月龄兔的耳软骨中分离出的唯一糖胺聚糖是4-硫酸软骨素和6-硫酸软骨素。这些硫酸软骨素通过木瓜蛋白酶消化从组织中分离出来时表现出分子量多分散性。硫酸软骨素在代谢上是异质的，因为放射性前体[¹⁴C]葡萄糖或[³⁵S]硫酸盐在体内和体外都优先掺入高分子量聚合物中。在体内15天期间未观察到放射性从高分子量硫酸软骨素转移到低分子量硫酸软骨素。提示组织中至少存在两个蛋白聚糖池。其中一个池具有代谢活性，而另一个则没有。
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